Research Announcements

Clinical Research Professionals Special Topics Seminar

“An Overview of Nebraska’s Response to Ebola”
Chris Kratochvil, MD and LuAnn Larson, BSN, RN, CCRP


“Exploring the Ethics of Isolation and Quarantine:  A Visit with Typhoid Mary”
Kristin Watkins, BA, MBA

June 9, 2015
University of Nebraska Medical Center, Sorrell Center, Room 1005

Clinical research professionals face logistical and ethical challenges on a daily basis. Ebola, a highly infectious disease, requires strict isolation procedures when caring for affected patients. For the past nine months, Dr. Kratochvil and his team of research professionals have been working on clinical trials with Ebola
patients and will share their experience of conducting research within a Biocontainment Unit. Ms. Watkins will use the historical example of the infectious disease typhoid fever as a framework for her one-woman play and facilitated discussion examining the social and ethical issues surrounding isolation and quarantine.

The relevant target audience for this presentation is all clinical research professionals.

Upon completion of this presentation, participants should be able to :
I. Describe the history of the Nebraska Biocontainment Unit as a clinical, training, and research enterprise.
2. Explain how the Nebraska Biocontainment Unit developed and implemented clinical research protocols for Ebola and standard research practices.
3. Identify how the Nebraska Biocontainment Unit continues to be involved with Ebola research initiatives at a national level.
4. Identify ethical issues surrounding isolation and quarantine
5. Describe factors that must be considered in terms of the patient, e.g. economic, social.
6. Operate in an ethical fashion towards patients’ concerns in situations of isolation and quarantine.
We invite all attendees to join us for the buffet supper. This activity, including the contact hours, is free-of charge to members of the Great Plains ACRP Chapter. Non-members are welcome and encouraged to attend.

Download Registration Information.

Please RSVP by June 8, 2015


New Western Blot Services available through Protein Structure Core Facility

The PSCF (Protein Structure Core Facility) is offering free hands-on individual training for our Peggy Simple Western Size or Charge system.  Training consists of three mornings, during which the trainee will run three plates of their own samples.  Our goal is that people leave training with an optimized lysate-antibody pair, and good confidence in operating the equipment and the associated software.

Our introductory pricing after training will be $10 per hour.  The PSCF currently provides all reagents other than lysate and primary antibody for standard size and charge runs.

Not familiar with what the Peggy can do?  It is manufactured by ProteinSimple, and performs automated Westerns or isoelectric focusing using capillaries.  Antibodies are used for detection by chemiluminescence.  Results can be viewed as electropherograms or lanes, and quantitation is imbedded in the software.  The goal is to save researchers time spent performing the manual steps of Western blot analysis, and to provide a method for determining post-translational modification of proteins (e.g. detecting phosphorylated and dephosphorylated signaling proteins). For more information, you can ask us or see the company’s website:

The PSCF still provides protein and peptide sequencing by Edman degradation, and protein quantitation by amino acid analysis.  These two methods are the gold standards for information about primary structure and amount of proteins.  For more information about the services offered in the PSCF, see,  e-mail us at, or call the director (Laurey Steinke, PhD) at 402-559-5176.  To schedule training, or arrange delivery of a sample, please call the PSCF (402-559-6647) and arrange a time with Michele Fontaine, Researcher.

Flow Cytometry Core Facility Director Canidate Seminar

Philip Hexley, Phd will present a seminar titled “Future Technology Developments in Cytometry”, August 14th, 2013 at 9:00 AM in the Durham Research Center, room 1005

Dr. Hexley is the Flow Cytometry Core Manager at Shriners Hospitals for Children in Cincinnati, Ohio.  See the campus calendar for details and to register for this seminar.